Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения Росс
发明人:
Замятнин Андрей Александрович (RU),Савватеева Людмила Владимировна (RU),Гороховец Неонила Васильевна (RU),Макаров Владимир Алексеевич (RU),Зерний Евгений Юрьевич (RU),Соловьев Андрей Геннадьевич (RU)
申请号：
RU2015105274
公开号：
RU2015105274A
申请日:
2015.02.17
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. A method of producing a purified, biologically active protein tritikaina-alpha - wheat protease, recombinant expression in a bacterial, yeast or plant system, characterized give the title belka.2. A method according to Claim. 1, wherein the protein is defined by SEQ ID №1, SEQ ID №2 SEQ ID №3 or SEQ ID №4.3. A method according to Claim. 1, wherein the host cell expression system selected from E.coli.4. A method according to Claim. 1, wherein the host cell expression system selected from P.pastoris.5. A method according to Claim. 1, wherein the host cell expression system is selected from Nicotiana sp.6. A method of producing a purified, biologically active protein recombinantly expressed in a host cell, comprising: a step of culturing a host cell transformed with a vector (for expression in Nicotiana sp - agroinfiltration or vectors based on plant viruses.) Containing DNA sequence encoding a protein as defined the sequence of SEQ ID №1, SEQ ID №2, SEQ ID №3 or SEQ ID №4 under conditions suitable for expression of the protein step of recovering expressed protein comprising separating the said protein from host cells to obtain the isolated protein preparation, purification of the protein preparation step carried out by methods hromatografii.7. A method according to claim. 1-6, characterized in that the built-alpha cDNA tritikaina protein may be modified by the leader peptide sequence and granulin-like domena.8. Purified biologically active protein preparation - recombinant truncated tritikain-alpha produced by the method of claim. 1, 2 and 6, a purity of not less than 85% and a yield of at least 55 mg per liter of bacterial kultury.9. The protein preparation - tritikain recombinant truncated alpha - cysteine protease1. Способ получения очищенного биологически активного белка тритикаина-альфа - протеазы пшеницы, рекомбинантно экспрессирующегося в бактериальной, дрожжевой или растительной системе, характеризующийся получением вышеназванного белка.2. Способ по п. 1,
