In-vitro fertilization (IVF) is a process of fertilization where an egg is combined with sperm outside the body, in vitro (in glass). The process involves monitoring and stimulating a womans ovulatory process, removing an ovum or ova (egg or eggs) from the womans ovaries and letting sperm fertilize them in a liquid in a laboratory. The fertilized egg (zygote) is cultured for 2-6 days in a growth medium and is then transferred to the same or another womans uterus, with the intention of establishing a successful pregnancy. Theoretically, IVF could be performed by collecting the contents from a womans fallopian tubes or uterus after natural ovulation, mixing it with sperm, and reinserting the fertilized ova into the uterus. However, -without additional techniques, the chances of pregnancy would be extremely small. The additional techniques that are routinely used in IVF include ovarian hyper stimulation to generate multiple eggs or ultrasound-guided transvaginal oocyte retrieval directly from the ovaries after which the ova and sperm are prepared, as well as culture and selection of resultant embryos before embryo transfer into a uterus. Humans are warm blooded and embryos formed in a womans fallopian tubes are consistently in an environment having 37°C temperature, a pH of 7.2 and osmolality of 1080. When such conditions are created in the IVF laboratory, highest pregnancy rates are achieved. Embryos are cultured in incubators which mimic the conditions in a human body. However during oocyte collection, ICSI, embryo manipulation, and embryo transfer these cells are exposed to extreme un-physiological conditions, like higher particle count, change of pH and osmolality. This is the main cause of IVF/ ICSI failure in the hands of the best embryologist. Laboratory culture conditions affect success rates of IVF/ICSI to a very large extent. Hence in dusty environments and with variable temperatures IVF success rates are low. To overcome these problems, this
