A system and method including a wand-type ultraviolet (UV) light assembly positioned above a keyboard and other computer peripheral device(s) to be sanitized. The UV light assembly is preferably moved across the peripheral device surface to eliminate nearly all germs, bacteria, viruses, pathogens and other microorganisms that have accumulated on the surfaces of the keyboard and other peripherals. Desirably, the system operation is managed by a controller device which is in communication with a host computer. In addition, the system allows for the collection and analysis of system performance data. The invention provides an extremely effective, compact, virtually cost-free and environmentally-friendly solution to disinfecting surfaces of keyboards and other peripheral devices.