Troels Kasper Høyer Scheel,Judith M. Gottwein,Tanja Bertelsen Jensen,Jens Bukh
申请号：
US13499788
公开号：
US09388389B2
申请日:
2010.10.01
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The present inventors developed hepatitis C virus recombinants expressing NS5A from genotype 1a, 1b, 2a, 3a, 4a, 5a, 6a or 7a in the context of a genotype 2a backbone. Additional recombinants express NS5A and the structural proteins (Core, E1 and E2), p7 and NS2 from genotype 1a, 1b, 3a, 4a, 5a, 6a or 7a in the genotype 2a backbone. Sequence analysis of the recombinants recovered after viral passage in Huh7.5 cells revealed adaptive mutations in NS5A and/or NS3. The importance of these mutations for improved growth kinetics was shown in reverse genetic studies.
