STEER, BRIAN,DYCAICO, MARK,KLINE, KATIE,TREFZER, AXEL,TODARO, TOM,SOLBAK, ARNE,EL-FARRAH, FATIMA,ALVARADO, ALBERTO,FREY, GERHARD
申请号：
ES13164378
公开号：
ES2548776T3
申请日:
2007.09.21
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
An isolated, synthetic or recombinant nucleic acid comprising (a) a nucleic acid sequence encoding a polypeptide having a phytase activity and having at least 95%, 96%, 97%, 98% or 99% or more identity of sequence with SEQ ID NO: 1, and having at least two, three, four, five, six, seven, eight, nine, ten, eleven, twelve or all 13 nucleotide modifications selected from the group consisting of: nucleotides at positions 139 to 141 of SEQ ID NO: 1 are replaced by TTT or TTC； nucleotides at positions 289 to 291 of SEQ ID NO: 1 are replaced by GTT, GTC, GTA or GTG； nucleotides at positions 289 to 291 of SEQ ID NO: 1 are replaced by GAA or GAG； nucleotides at positions 406 to 408 of SEQ ID NO: 1 are replaced by CAT or CAC； nucleotides at positions 475 to 477 of SEQ ID NO: 1 are replaced by GTT, GTC, GTA or GTG； nucleotides at positions 475 to 477 of SEQ ID NO: 1 are replaced by GAA or GAG； nucleotides at positions 487 to 489 of SEQ ID NO: 1 are replaced by CGT, CGC, CGA, CGG, AGA or AGG； nucleotides at positions 490 to 492 of SEQ ID NO: 1 are replaced by CGT, CGC, CGA, CGG, AGA or AGG； nucleotides at positions 502 to 504 of SEQ ID NO: 1 are replaced by CGT, CGC, CGA, CGG, AGA or AGG； nucleotides at positions 535 to 537 of SEQ ID NO: 1 are replaced by CGT, CGC, CGA, CGG, AGA or AGG； nucleotides at positions 676 to 678 of SEQ ID NO: 1 are replaced by GAT or GAC； nucleotides at positions 697 to 699 of SEQ ID NO: 1 are replaced by TGG； nucleotides at positions 823 to 825 of SEQ ID NO: 1 are replaced by GTT, GTC, GTA or GTG； nucleotides at positions 865 to 867 of SEQ ID NO: 1 are replaced by GCT, GCC, GCA or GCG； and nucleotides at positions 1045 to 1047 of SEQ ID NO: 1 are replaced by TAT or TAC； where the polypeptide has improved thermostability over the phytase of SEQ ID NO: 2, and retains 100% of the activity after treatment at 80 ° C for 30 minutes； (b) a nucleic acid encoding a polypeptide that has a phytase activity and that has at least 95%, 96%, 97%, 98% or 99% or more
