Kurkin Vladimir Aleksandrovich (RU),Куркин Владимир Александрович (RU),Kupriyanova Elena Aleksandrovna (RU),Куприянова Елена Александровна (RU)
申请号：
RU2018142704
公开号：
RU0002701726C1
申请日:
2018.12.03
申请国别(地区):
RU
年份:
2019
代理人:
摘要：
FIELD: medicine； pharmaceuticals.SUBSTANCE: invention refers to chemical-pharmaceutical industry, namely to a method for quantitative determination of flavonoids in black poplar leaves. Method for quantitative determination of flavonoids in black poplar leaves, consisting in preliminary production of water-and-alcohol extraction from vegetable raw material by extraction of 1 g of a precise weighed portion of ground raw material ground to particle size of 1 mm with 70 % ethyl alcohol in terms of a substance of flavonoid nature, by differential spectrophotometry in relation "raw material-extractant" – 1:30, wherein flavonoids are determined at wavelength of 414 nm in terms of routine and absolutely dry material is calculated by formulawhere x is content of sum of flavonoids in terms of rutin, %； D is the optical density of the test solution； Do is the optical density of the solution of the State standard routine sample； m is weight of raw material, g； mo is mass of State standard sample of rutin, g； W is weight loss during drying, %； in case of absence of standard routine sample, it is reasonable to use theoretical value of its specific absorption coefficient equal to 240,where x is content of flavonoids sum in recalculation for routine, %； D is the optical density of the test solution； m is weight of raw material, g； 240 is specific absorption coefficient (E1%1cm) of the state standard routine at 414 nm； W is weight loss during drying, %.EFFECT: method for quantitative determination of flavonoids in black poplar leaves is effective.1 cl, 3 dwg, 3 tbl, 2 exИзобретение относится к химико-фармацевтической промышленности, а именно к способу количественного определения флавоноидов в листьях тополя черного. Способ количественного определения флавоноидов в листьях тополя черного, заключающийся в предварительном получении водно-спиртового извлечения из растительного сырья путем экстракции 1 г точной навески измельченного до размера частиц 1 мм растительного сырья 70%-ным эти
