PROBLEM TO BE SOLVED: To efficiently redifferentiate a transformed plant body.SOLUTION: The method for forming a gene transferred plant body includes: transferring an agrobacterium into a tissue of a leaf blade by returning the leaf blade of a leaf having a leafstalk such as Jatropha curcas in a state having the leafstalk, and immersing with a fungus liquid of the agrobacterium from the state of more decompressed state than atmospheric pressure to pressure of the atmospheric pressure then contacting an antibiotic and the agrobacterium in the tissue of the leaf blade by immersing the leaf blade into an aqueous solution of the antibiotic and then contacting the leaf blade with a bactericide other than the antibiotic such as a hypochlorous acid. After that, callus induction is performed on a culture medium including the antibiotic using a part of the leaf blade into which the agrobacterium is transferred as a tissue fragment.COPYRIGHT: (C)2012,JPO&INPIT【課題】形質転換した植物体を効率よく再分化させる。【解決手段】ヤトロファ・クルカスなどの有柄葉の葉身を、葉柄を備えた状態でアグロバクテリウムの菌液に浸漬した状態で、大気圧より減圧した状態から大気圧に戻すことにより葉身の組織にアグロバクテリウムを導入する。その後、葉身を抗生物質の水溶液に浸漬して抗生物質と葉身の組織中のアグロバクテリウムを接触させ、次いで次亜塩素酸などの抗生物質以外の殺菌剤と接触させる。その後、アグロバクテリウムが導入された葉身の部分を組織片として、抗生物質を含む培地上でカルス誘導を行う。【選択図】図1
