The invention relates to a method to increase growth or differentiation in vitro in a cell or tissue culture comprising culturing said cell(s) or tissue and applying pulsed radiofrequency (PRF) to said cell or tissue. In said method the cells or tissue are derived from a plant or an animal (including human) and preferably the cells are undifferentiated cells. Also part of the invention is a method as described herein wherein the cells or tissue are derived from a plant and in which differentiation implies the outgrowth of roots and/or shoots or of somatic embryos. The invention further comprises the use of PRF for increasing the growth or differentiation inin vitrocell or tissue culture.The invention also comprises a cell or tissue culture treated according to a method according to the invention. Preferably such a cell or tissue culture is derived from a plant. Then, in yet another embodiment, the invention is directed to a plant grown from such a cell or tissue culture. Alternatively, the invention comprises a cell culture that is treated according a method of the invention wherein the cells are derived from an animal, preferably a human.本發明係關於增進細胞或組織培養物中的試管內生長或分化之方法，其包含培養該細胞或組織，且向該細胞或組織施加脈衝式射頻(PRF)。在該方法中，細胞或組織係源自植物或動物(包括人類)，且細胞較佳為未分化細胞。又，本發明之一部分為如本文所述之方法，其中細胞或組織係源自植物，且其中分化意指根及/或芽長出或體細胞胚長出。本發明進一步包含PRF增進細胞或組織培養物中的試管內生長或分化之用途。本發明亦包含根據本發明之方法處理之細胞或組織培養物。此類細胞或組織培養物較佳係源自植物。繼而，在另一具體實例中，本發明係針對自此類細胞或組織培養物生長之植物。或者，本發明包含根據本發明之方法處理之細胞培養物，其中細胞係源自動物，較佳源自人類。
