The application provides fully human monoclonal antibodies directed against member 4 of the tumor necrosis factor receptor superfamily 4 (TNFRSF4) (also known as OX40 and CD134). The application also provides a method for generating hybridomas using humanized transgenic rats, a nucleic acid molecule encoding an anti-OX40 antibody, an expression vector for expressing the anti-OX40 antibody, and a host cell. The invention also provides a method for verifying antibody function in vitro and antibody efficacy in vivo. The antibodies of the invention provide very effective agents for treating a variety of cancers by modulating human immune function.本申請提供了針對腫瘤壞死因數受體超家族的成員4 (TNFRSF4)(也稱為OX40和CD134)的全人單株抗體。本申請還提供了使用人源化轉基因大鼠產生雜交瘤的方法、編碼抗OX40抗體的核酸分子、用於表達抗OX40抗體的表達載體和宿主細胞。本發明還提供了用於體外驗證抗體功能和體內抗體功效的方法。本發明的抗體提供了用於藉由調節人免疫功能治療多種癌症的非常有效的藥劑。
