In the present invention, young leaves of a new branch were collected, disinfected and injured, and callus and adventitia were induced, and shoots were directly formed therefrom to differentiate into young plants. , And a method of producing seedlings of bivalve bifidus cerevisiae "Gicera 5" was established and wounded at the center of leaf by the dentition method of the leaf slice (leaf piece), the lower side of the leaf was directed upward, It was confirmed that callus formation was remarkably increased when the seeds were in contact with each other. It was confirmed that the production efficiency of the culture seedlings was increased when cultured in callus induction medium, young plant differentiation medium and plant propagation medium according to the present invention, The cultivation method can be useful for the mass production of the bichalidaceae kernel "Gicera 5".본 발명에서 신초(새가지)의 어린 잎을 채취하여 소독 및 치상한 후, 캘러스 및 부정아를 유도하였고, 이로부터 직접 신초를 형성하여 어린 식물체로 분화시켰으며, 이를 기외 순화시켜, 유목으로부터 성목을 생산하여, 양앵두 왜성대목 “기세라 5”의 묘목을 제조하는 방법을 확립하였고, 상기 잎 절편(엽편)의 치상 방법으로 잎 중앙맥에 상처를 내고, 잎 아랫면이 위를 향하고, 잎 윗면이 배지에 닿게 치상하였을 때, 캘러스의 형성이 현저하게 증가함을 확인하였으며, 본 명의 캘러스 유도 배지, 어린 식물체 분화 배지, 식물체 증식 배지에서 배양하였을 때, 배양묘의 생산 효율이 증가함을 확인하여, 본 발명의 배양방법은 양앵두 왜성대목 “기세라 5”의 대량생산에 유용하게 사용될 수 있다.
