Devices for determining the concentration of an analyte in a fluid are provided. Also provided are systems and kits for use in practicing the subject methods. Fluid handling devices for determining the concentration of an analyte in a fluid are provided. Embodiments of the subject fluid handling devices include a skin piercing element, at least one analyte sensing chamber and a pumping chamber having a deflectable membrane, wherein the pumping chamber is capable of transporting fluid from the skin piercing element to a sensing chamber upon deflection of the deflectable membrane. Also provided are novel kits that include the subject devices.
