FEDERALNOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE NAUKI NAUCHNO-ISSLEDOVATELSKIJ INSTITUT FIZIKO-KHIMICHESKOJ MEDITSINY FEDERALNOGO MEDIKO-BIOLOGICHESKOGO AGENTSTVA (FGBUN NII FKHM FMBA ROSSII)
发明人:
BELOVA OLGA VLADIMIROVNA,Белова Ольга Владимировна,SERGIENKO VALERIJ IVANOVICH,Сергиенко Валерий Иванович,ARION VITALIJ JAKOVLEVICH,Арион Виталий Яковлевич,LUKANIDINA TATJANA ABRAMOVNA,Луканидина Тать
申请号：
RU2011126310
公开号：
RU2481113C2
申请日:
2011.06.28
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to medical biotechnology and pharmaceutical industry, and concerns a method for preparing the substances having an effect on proliferation of the A431 human epidermoid carcinoma cells. The described method involves grinding, homogenising of skin, thermodenaturation of the supernatant, cooling of the mixture, centrifugation of the denatured material, processing by cooled acetone, washing of the precipitate, drying, dissolution of the precipitate in 0.005-0.015 M solution of tris-HCl+0.15M NaCl, and centrifugation, followed by chromatography on a Sephadex G-50 column and collection of the end substance of molecular weight 1400 to 15000 Da, debilitation and lyophilisation, with chromatography simultaneously with a substance of molecular weight 1400 to 15000 Da involving collection of the substance of molecular weight more than 15000 Da, re-chromatography of the substance of molecular weight more than 15000 Da on the Sephadex G-50 column followed by collection of two substances: one substance has molecular weight more than 15000 Da, and the second one has molecular weight 1400 to 15000 Da both substances are desalted and lyophilised, and the substances of molecular weight 1400 to 15000 Da prepared by re-chromatography is combined with the substance of the same molecular weight prepared after the first chromatography.EFFECT: invention may be used to prepare the drugs for treating skin diseases, as well as the agents for immune correction.2 dwg, 4 tbl, 2 exИзобретение относится к области медицинской биотехнологии и фармацевтической промышленности и касается способа получения веществ, влияющих на пролиферацию эпидермоидных клеток карциномы человека А431. Описанный способ включает измельчение, гомогенизацию кожи, термоденатурацию супернатанта, охлаждение смеси, центрифугирование денатурированного материала, обработку охлажденным ацетоном, отмывку выпавшего осадка, высушивание, растворение осадка в 0,005-0,015 М раст
