A therapeutic composition comprising a yeast expressing at least one heterologous intracellular antigen from an infectious pathogen or a disease and at least one heterologous extracellular antigen from the pathogen or the disease wherein the intracellular antigen is a different antigen than the extracellular antigen, use of the compositions in eliciting an antigen-specific cell-mediated and/or humoral immune response in an individual or in treating or preventing infection with the pathogen or for treating or preventing the disease, and a method to produce a therapeutic composition for eliciting a cell-mediated and/or a humoral immune response against an infectious pathogen or a disease.
