The present invention is a grafted seedling producing apparatus 10 capable of rotating seedlings W in an alignment direction even if they are those having large leaves and thereafter halting the rotation to align them, or the apparatus 10 having loading portions 1, 2 which are provided with a seedling carry-in mechanism 11 for sequentially transporting a tray 7 of a large number of arranged seedlings W, a grip hand 12 for individually gripping stems of seedlings on the seedling carry-in mechanism 11, a cutter mechanism 23 for individually cutting the stems of seedlings gripped by the grip hand 12, an alignment guide 14 for aligning seedlings to be transported by the grip hand 12, a roller 17 for rotating the seedlings W to an alignment direction at the side of the seedling carry-in mechanism 11 from the alignment guide 14, and a transport mechanism 13 for moving the grip hand 12 to the alignment guide side a retaining mechanism 15 provided with to a main alignment member 16 which regulates a direction to which the seedling leaf of a seedling W gripped by the grip hand 12 at the loading portions 1, 2 faces to the alignment guide 14 and temporarily retaining the seedlings W transported from the grip hand 12 and an attachment treatment portion 5 for attaching the seedlings W delivered from the retaining mechanism 15.