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DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION
专利权人:
发明人:
ALTROGGE LUDGER,GLEISSNER TIMO,HEINZE ANDREAS,HERMSMEIER SVEN
申请号:
IN201617036329
公开号:
IN201617036329A
申请日:
2016.10.24
申请国别(地区):
IN
年份:
2017
代理人:
摘要:
The invention relates to a device for applying an electric field to a suspension of cells comprising at least one chamber which comprises at least one internal space (40) for holding the suspension the internal space (40) comprising at least two segments (41 42) wherein each segment (41 42) comprises at least one electrode (43 44) and wherein neighboring electrodes (43 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46) and wherein the edges of the electrodes (43 44) facing each other within the internal space (40) are rounded. Rounding the electrodes edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method wherein voltage is applied to at least one active electrode (43 44) while the electrodes (43 44 45) or electrode segments next and/or opposite to the active electrode (43 44) are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.
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