Provided are a method of maintaining the ovaries of marine fish, and a method of obtaining fertilized eggs. Provided is a method of maintaining ovaries in which the ovaries are extracted from marine fish and the ovaries or fragmented ovaries are cultured in a culture medium. Also, provided is a method of obtaining fertilized eggs of marine fish, the method being characterized in that ovaries, which have been maintained after extraction, are allowed to ovulate and are subjected to artificial insemination. Further, the present invention relates to a method of maintaining the ability of ovulated eggs of marine fish to fertilize by using the culture medium. According to the present invention, fertilized eggs of marine fish can be easily obtained and seed can be efficiently produced.
