AbstractA NOVEL ANTIBIOTIC COMPRISING AN ANTIBODY MIMETIC, PREPARATIONMETHODS AND USES THEREOFThe present invention belongs to field of biology and medicine, and especially relates to a novel antibiotic comprising a mimetic antibody, its preparation methods and uses thereof. The novel antibiotic comprising comprising an antibody mimetic and a colicin, or comprising an antibody mimetic and a channel-forming domain of a colicin, the antibody mimetic covalently bonded to the carboxyl end of the polypeptide of the colicin or the channel-forming domain of the colicin, wherein the colicin is selected from a group consisting of colicin El, Ia, Ib, A, B, N wherein said antibody mimetic being yielded by fusing two complementarity determining regions (CDRs), VHCDRI andVLCDR through a cognate framework region (VHFR2) of an immunoglobulin wherein the immunoglobulin specifically recognizes bacterium porins. Its antibacterial ability is a thousandfold powerful than normal antibiotics. Due to its unique action mechanism, drug resistance resulted in mutation can hardly be acquired by pathogenic bacteria. And the antibiotic will not hurt normal human cells when it kills pathogenic bacteria. Therefore, it can be used for manufacturingantibacterial medicament of killing Dipococcus intracellularis, vancomycin-resistant enterococci, Methicillin-resistant Staphylococcus aureus, or multidrug resistant Pesudomonas aeruginosa.
