PIROGOV RUSSIAN NATIONAL RESEARCH MEDICAL UNIVERSITY (RNRMU)
发明人:
PETRAKOVA, Olga Sergeevna,BORISOV, Mikhail Aleksandrovich,VOROTELYAK, Ekaterina Andreevna,GVAZAVA, Inessa Givievna,ROGOVAYA, Olga Sergeevna,VASILIEV, Andrey Valentinovich,BOGDANOVA, Ekaterina Andreevn
申请号：
SG11201907086W
公开号：
SG11201907086WA
申请日:
2018.02.08
申请国别(地区):
SG
年份:
2019
代理人:
摘要：
The technical result of the invention is to simplify the technology of obtaining insulin-producing cells, obtaining at least 70% of functionally active insulin-producing cells in cell culture, that underwent differentiation. The method comprises obtaining epithelial progenitor cells and their subsequent differentiation into pancreatic cells, capable to glucose-sensitive insulin secretion in which pancreatic differentiation is performed in two stages: (a) at the first stage cells are differentiated within 4-15 days in a culture medium containing at least serum of a mammal, glutamine, epidermal growth factor, transferrin, sodium selenite, retinoic acid, isoproterenol； (b) at the second stage, cells are differentiated within 4-15 days in a culture medium containing at least serum of a mammal, glutamine, epidermal growth factor, retinoic acid, nicotinamide, hepatocyte growth factor, dexamethasone； moreover, the cultivation in both stages is carried out in gas atmosphere of 5% CO2 at 37°C. Group of inventions includes cell product of insulin-producing cells of a mammal, and a method of differentiation of pancreatic epithelial progenitor cells of mammals, including humans, as well as a method for replacement therapy of diabetes mellitus using cell product.
