On the basis of the present invention, D-(+)-cycloserine (D-(+)-Cycloserine), an antibiotic such as LPS-stimulated mouse macrophage cell line (RAW 264.7 cells) has NO production inhibitory activity on the basis of D-( Disclosed is an anti-inflammatory composition using antibiotics such as +)-cycloserine (D-(+)-Cycloserine).본 발명은 D-(+)-사이클로세린(D-(+)-Cycloserine) 등의 항생제가 LPS로 자극된 마우스 대식세포주(RAW 264.7 cells)에서 NO 생성 억제 활성을 가짐에 기초하여, D-(+)-사이클로세린(D-(+)-Cycloserine) 등의 항생제를 이용한 항염증용 조성물을 개시한다.