METHOD FOR CONSTRUCTING GLRX1 GENE KNOCK-OUT ANIMAL MODEL BASED ON CRISPR/CAS9

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专利权人:

NANJING AGRICULTURAL UNIVERSITY

发明人:

LI, Chunbao,ZHOU, Guanghong,ZOU, Xiaoyu,SHI, Xuebin,XU, Xinglian

申请号：

WO2018CN85432

公开号：

WO2018219093(A1)

申请日:

2018.05.03

申请国别(地区):

世界知识产权组织国际局

年份:

2018

代理人:

摘要：

The invention discloses a method for constructing a Glrx1 gene knock-out animal model based on CRISPR/Cas9, comprising the steps of: 1. selection and design of sgRNA targeting Glrx1 gene of mice; 2. construction of an sgRNA vector; 3. in vitro transcription of sgRNA; 4. injection of mouse fertilized eggs; 5. birth and identification of F0-generation mice; and 6. breeding of positive F0-generation mice, and birth and identification of F1-generation mice.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/