Method and device for predicting optimum insemination time of an animal and/or pregnancy detection, by determining in a body fluid sample of said animal the presence and/or concentration of at least one analyte of interest selected from the group comprising progesterone, lutenizing hormone LH, follicle stimulating hormone FSH, prolactin PRL, sex hormone binding globuline SHBG, estradiol and/or testosterone, wherein a lateral flow immunoassay is used.
