PROBLEM TO BE SOLVED: To provide fat tissue-derived mesenchymal stem cells which are high in quality and more suitable for transplantation, for clinically applying the fat tissue-derived mesenchymal stem cells useful as a medical material.SOLUTION: About fat tissue-derived mesenchymal stem cells obtained by culturing under low-serum condition, expression of a tissue factor is detected by flow cytometry and an average fluorescent intensity ratio is compared with a reference value. When the ratio is lower than the reference value, it is determined that they are cells with high quality.SELECTED DRAWING: Figure 4COPYRIGHT: (C)2016,JPO&INPIT【課題】医療用材料として有用な脂肪組織由来間葉系幹細胞の臨床応用を図るべく、移植に一層適した高品質の脂肪組織由来間葉系幹細胞を提供することを課題とする。【解決手段】低血清条件下で培養して得られた脂肪組織由来間葉系幹細胞について組織因子の発現をフローサイトメトリーで検出し、平均蛍光強度比を基準値と比較する。基準値よりも低い値を示した場合に高品質の細胞であると判定する。【選択図】図4
