#$%^&*AU2019395241B120201001.pdf#####Abstract Disclosed relates to a preparation method and application of antibacterial active substances of Bacillus pumilus. A Bacillus pumilus BP strain was collected in the China General Microbiological Culture Collection Center on March 21, 2019, Address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; Collection Number: CGMCC No.17424. Disclosed also relates to antibacterial active substances prepared by using the strain and relevant application. Three antibacterial active fatty alcohol compounds are first extracted from Bacillus pumilus, but are not found in the existing Bacillus pumilus.