BAUMANN, Ute,CIGAN, Andrew Mark,PALLOTTA, Margaret Anne,SINGH, Manjit,SUCHECKI, Radoslaw,WATSON-HAIGH, Nathan Spencer,WHITFORD, Ryan
申请号：
AU2018385522
公开号：
AU2018385522A1
申请日:
2018.12.10
申请国别(地区):
AU
年份:
2020
代理人:
摘要：
Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant produced using the methods and compositions described herein.
