Общество с ограниченной ответственностью г.Санкт-Петербург "Покровский банк стволовых клеток" (RU)
发明人:
Смирнова Наталья Владимировна (RU),Смолянинов Александр Борисович (RU),Хрупина Александра Сергеевна (RU),Михайлов Виктор Васильевич (RU),Хурцилава Отари Гивиевич (RU),Силин Алексей Викторович (RU),Рун
申请号：
RU2011116021/15
公开号：
RU2011116021A
申请日:
2011.04.22
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
1. A method for preventing periimplantitis when installing the dental implant, which consists in carrying out a therapeutic effect on the tissue surrounding the installation site of the dental implant with the local administration of stem cells, characterized in that before carrying out the prevention periimplantitis 2-3 weeks prior to implant installation produce fence adipose tissue of the patient, preferably from his abdominal area in an amount of 10-100 ml, isolated therefrom stromal-vascular fraction cells after treatment with the multistage PIC leduyuschim their culturing and isolating the adhesive to the culture plastic fraction mesenchymal stem cells of adipose tissue, after separation of the latter is carried immunophenotype of the cultured cell analysis by flow cytometry and karyological analysis of cultured cells with the identification of genetic instability markers, therapeutic intervention in the form of oral hygiene antimicrobial, antiinflammatory and strengthening therapy is carried out in the period of culturing stem millstands to adipose tissue prior to implantation to prepare the graft in the patient produce fence 20 ml of peripheral blood alikvatiruyut it in two test tubes of 10 ml, mixed with 10% anticoagulant CPDA-1 and centrifuged them with an acceleration of 300 g for 10 min to obtain plasma, comprising leukocyte-platelet suspension, with the formation of the "a" samples are collected 1 ml rich plasma with thrombin, were added to 300 .mu.l of calcium gluconate and the mixture was incubated for 15 min at t 37 ° C, more sample "a" and the remaining plasma was centrifuged at 640 g for 10 min, and the odds1. Способ профилактики периимплантита при установке дентального имплантата, заключающийся в осуществлении терапевтического воздействия на ткани вокруг места установки дентального имплантата с локальным введением стволовых клеток, отличающийся тем, что перед осуществлением профилактики периимплантита за 2-3 недели до установки имплантата производ
