This invention is directed to the preparation and use of stromal cells for treatment of cardiac tissue. The method for preparing stromal cells comprising: a) enriching stromal progenitor cells from a bone marrow sample； b) culturing said stromal progenitor cells in a culture vessel to expand stromal cells, wherein the stromal cells adhere to the culture vessel； c) detaching the adhered stromal cells of step (b) and incubating them ill an enhanced surface roller bottle； and d) harvesting the stromal cells from said roller bottle.
