The present invention relates to a Cistanche tubulosa and fucoidan extract having effects of preventing hair loss and promoting hair growth and a manufacturing method of the same and more specifically, to a Cistanche tubulosa and fucoidan extract for preventing hair loss and promoting hair growth and a manufacturing method of the same wherein the extract contains at least one extract as an active ingredient among: an extract, as a phenylethanoid glycoside, including at least one among echinacoside, acteoside, and a sulfuric acid group; and an extract including at least one among the phenylethanoid and the sulfuric acid group. [Reference numerals] (AA) Seaweed(Undaria pinnatifida sporophyll/Laminaria japonica); (BB) Pulverizing; (CC) Extract acid and hot water; (DD) Separate solid-liquid; (EE) Supernatant neutralization; (FF) Diatom filtration; (GG) Ultrafilter; (HH) Alcohol immersion; (II) Centrifugation; (JJ) Drying; (KK) Packaging; (LL) Alginic acid separation by depositing; (MM) 10N NaOH solution; (NN) Three times capacity of ethanol is added본 발명은 탈모예방 및 발모촉진 효과를 갖는 육종용 및 후코이단 추출물 및 이의 제조방법에 관한 것으로, 페닐에타노이드 배당체로서 에키나코사이드, 악테오사이드 및 황산기 중 적어도 어느 하나를 포함한 추출물 및 페닐에타노이드 배당체 및 황산기 중 적어도 어느 하나를 포함한 추출물 중 적어도 어느 하나의 추출물을 유효성분으로 하는 탈모예방 및 발모촉진 효과를 갖는 육종용 및 후코이단 추출물 및 이의 제조방법에 관한 것이다.