Федеральное государственное бюджетное образовательное учреждение высшего образования Санкт-Петербургская государственная академия ветеринарной медицины (RU)
发明人:
Сухинин Александр Александрович (RU),Герасимов Сергей Вадимович (RU),Макавчик Светлана Анатольевна (RU)
申请号：
RU2016129877
公开号：
RU2016129877A
申请日:
2016.07.20
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Method for the preparation of aluminum oily vaccine against campylobacteriosis is proposed. Proposed method includes steps of isolating and accumulating causative agent of Campylobacter fetus subspecies fetus Campylobacter fetus on a nutrient medium, washing out accumulated colonies of the pathogen with 0.9 % NaCl solution, inactivating the accumulated biomass with teotropin in a ratio of 0.3 % of teotropine to 100 % of the biomass with subsequent combination of inactivated biomass with adjuvant aluminum hydroxide and introduction into the oil adjuvant. Vaccine obtained by proposed method has following component ratio (mass%): 0.14% suspension of Campylobacter serogroup cells fetus subspecies fetus in culture medium with a concentration of 2×10CFU */cm； 26.81 % of 0.9 % NaCl solution； 0.08 % of teotropine； 2.97 % of a 2 % suspension of AL(OH)； rest is oil adjuvant.EFFECT: proposed method is effective and safe and can be used in biotechnology to produce a vaccine against campylobacteriosis.1 cl, 3 tbl, 1 ex
