PROBLEM TO BE SOLVED: To provide a determination method of a pore distribution state capable of conveniently and accurately determining a distribution of pores, and determination method of an amount of nanoparticles absorbed from the pores.SOLUTION: A distribution state of pores on a skin surface is determined by: applying phosphor-supporting nanoparticles to a skin surface, the phosphor-supporting nanoparticles formed by causing nanoparticles formed of biocompatible polymer to support phosphors at least at one of the inside and the surface thereof and observing them using a fluorescence video microscope to determine the distribution state of the pores on the skin surface.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2019,JPO&INPIT【課題】毛穴の分布を簡便に且つ精度良く判定可能である毛穴分布状態の判定方法、および毛穴からのナノ粒子の吸収量の判定方法を提供する。【解決手段】生体適合性高分子で形成されたナノ粒子の内部または表面の少なくとも一方に蛍光物質を担持させた蛍光物質担持ナノ粒子を皮膚表面に塗布した後、蛍光ビデオマイクロスコープを用いて観察することにより皮膚表面の毛穴の分布状態を判定する。【選択図】図1
