A corneal preservation and rehydration device includes a box body and a box lid (1), wherein the box body and the box lid are detachably connected. A corneal bracket (2) is provided in the box body for holding a cornea, wherein a surface of the corneal bracket for placing a cornea is a convex spherical surface (21), and a blocking portion (22) is arranged at the surrounding of the convex spherical surface (21) for preventing a cornea from dropping out.