A method for synchronizing ovulation in Buffalo by sequential injection of hormones is disclosed. PROVAGO which consists Buserelin Acetate is administered to an open Buffalo during an estrous cycle in order to stimulate follicle development. SUDAMATE which contains Cloprostenal Sodium is then administered to initiate corpus luteum regression about five to eight days after administration of PROVAGO. A second dose of PROVAGO is then administered concomitantly with SUDAMATE injection or up to about three days after SUDAMATE injection. This second dose of PROVAGO functions to stimulate the ovulation of a dominant follicle and the Buffalo is then breed within one day of the administration of the second dose of PROVAGO.
