The invention describes an anchoring system for a stretcher (1), comprising: a pair of horizontal rails (2) coupleable to an ambulance floor； a pair of first auxiliary wheels (3) fixed to a front portion of a frame (11) of the stretcher (1) and coupleable to said pair of horizontal rails (2)； a pair of second auxiliary wheels (4) fixed to the frame (11) in a position longitudinally adjacent to, and aligned with, the corresponding first auxiliary wheel (3), and they are also coupleable to said pair of horizontal rails (2). Furthermore, the profile of the rails (2) comprises an upper horizontal segment (23), such that when the auxiliary wheels (3, 4) are introduced into the rails (2), they are confined between the lower horizontal segment (21) and the upper horizontal segment (23) of said rails (2), preventing any vertical movement thereof.
