BÖTTNER, Mewes,LANG, Christine,VEEN, Markus,SCHILLING, Michael,REINDL, Andreas
申请号：
ES09761379
公开号：
ES2574135T3
申请日:
2009.05.19
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
Inactive form of a microorganism belonging to the genus Lactobacillus, the microorganism being selected from the group consisting of microorganisms that have the registration number of DSMZ DSM 19825, DSM 19826 or DSM 19827, characterized in that the inactivated form means a dead or inactivated cell that no longer it can form an individual colony on a specific plate for microorganisms belonging to the genus of Lactobacillus in which said dead or inactivated cell has a cell membrane either intact or broken and the microorganism shows the following property (b) when subjected to the next test (a): Test (a): (a) the microorganism is grown for 24 h at 37 ° C under anaerobic conditions in a synthetic medium containing 15 g / l peptides with an initial cell density of 1x107 10 cells / ml, in which the Synthetic medium has the following composition: guanine: 0.1 g / l cytosine: 0.1 g / l thymidine: 0.1 g / l 2'-deoxyadenosine: 0.1 g / l 2'-deox iuridine: 0.1 g / l K2HPO4: 2 g / l sodium acetate: 5 g / l MgSO4 heptahydrate: 0.1 g / l diammonium hydrogen carbonate: 2 g / l CaCl2 dihydrate: 0.5 g / l oleic acid : 0.1% (w / v) cyanocobalamin: 0.02 mg / l riboflavin: 10 mg / l folic acid: 0.2 mg / l 5-pyridoxal phosphate monohydrate: 10 mg / l 4-aminobenzoic acid: 0 , 2 mg / l D - (+) - biotin: 1 mg / l ascorbic acid: 500 mg / l nicotinic acid: 10 mg / l pantothenate Ca: 10 mg / l thiamine: 1 mg / l cobalt nitrate (II ) hexahydrate: 500 mg / l MnSO4 monohydrate: 20 mg / l MgSO4 heptahydrate: 500 mg / l Na2MoO4: 0.04 mg / l PTU extract: 15 g / l or 7 g / l D-glucose monohydrate: 10 g / l ； (b) the cells are removed by centrifugation at 4000 x g for 15 min； and (c) the concentration of peptides in the resulting supernatant is determined； Property (b): The microorganism leads to a reduction in the concentration of peptides in the culture medium so that the concentration of peptides in the supernatant after incubation for 24 h is reduced by at least 20% compared to the concentratio
