MANNOSIDASES CAPABLE OF UNCAPPING MANNOSE-1-PHOSPHO-6-MANNOSE LINKAGES AND DEMANNOSYLATING PHOSPHORYLATED N-GLYCANS AND METHODS OF FACILITATING MAMMALIAN CELLULAR UPTAKE OF GLYCOPROTEINS
The invention provides mannosidases capable of uncapping mannose-l-phospho- 6-mannose moieties and demannosylating phosphorylated N-glycans, methods of using such mannosidases, glycoproteins produced using the methods, as well as methods of facilitating mammalian cellular uptake of glycoproteins.Cette invention concerne des mannosidases capables déliminer la coiffe de fragments mannose-1-phospho-6-mannose et de démannosyler les N-glycanes phosphorylés, des procédés dutilisation desdites mannosidases, des glycoprotéines produites par ces procédés, ainsi que des méthodes facilitant lassimilation des glycoprotéines par des cellules de mammifères.
