Provided are a method for producing cell aggregates containing a ciliary marginal zone-like structure, etc., said method being characterized by comprising a step for culturing cell aggregates containing a retinal tissue, in said retinal tissue Chx10-positive cells existing at a ratio of 20% or greater, in a serum free medium or a serum medium containing a Wnt signaling pathway agonist only for a period until RPE65 gene-expressing cells appear, and then culturing "cell aggregates in which no RPE65 gene-expressing cell appears" in a serum free medium or a serum medium containing no Wnt signaling pathway agonist. According to the present invention, a ciliary marginal zone-like structure can be produced at a high efficiency.
