Needle assemblies for use in ablation procedures include a needle having an electrically conductive portion and at least one conductive member extending at least partially through a bore of the needle. A portion of the at least one conductive member is physically and electrically connected to the electrically conductive portion of the needle. Ablation systems and methods of ablation may include such needle assemblies. Methods of forming needle assemblies for use in ablation procedures include disposing at least one conductive member within a needle and physically and electrically connecting the at least one conductive member to an electrically conductive portion of the needle.