THE INVENTION RELATES TO AN ANTI-CANCER AGENT WHEREIN THE ANTI-CANCER AGENT COMPRISES OF A BIOLOGICALLY ACTIVE COMPOUND OF CO-II (17βH-NERIIFOLIN) WHICH IS A CARDENOLIDE GLYCOSIDE AND IS ISOLATED FROM THE LEAVES OF CERBERA ODOLLAM. ACCORDINGLY, BIOLOGICALLY ACTIVE COMPOUND OF CO-II (17βH-NERIIFOLIN) IS EXTRACTED AND ISOLATED WITH THE FOLLOWING STEPS OF (A) HEAT-TREATING LEAVES OF CERBERA ODOLLAM (B) GRINDING THE LEAVES FROM STEP (A): (C) EXTRACTING THE BIOLOGICALLY ACTIVE COMPOUND FROM THE GRINDED LEAVES (D) PARTITIONING THE EXTRACT FROM STEP (C) (E) FRACTIONATING THE PARTITIONED EXTRACT (F) PURIFYING THE FRACTIONS FROM STEP (E) AND (G) ELUTING THE PURIFIED FRACTIONS TO OBTAIN THE BIOLOGICALLY ACTIVE COMPOUND. THE BIOLOGICALLY ACTIVE COMPOUND OF CO-II EXHIBITS STRONG CYTOTOXIC ACTIVITIES AGAINST OVARIAN CANCER CELL LINES OF SKOV-3 AND CAOV3.
