A positioning tool aids in the positioning of at least one instrument at a subcutaneous treatment site visible via a medical imaging device. The positioning tool includes a penannular member, at least one spacing member having a proximal end attached to the penannular member and a distal end spaced from the proximal end, and at least one aligning portion attached to the at least one spacing member. The aligning portion is configured for aligning the tool with a target visible via the medical imaging device. A kit includes a plurality of the tools. A method includes use of the tool.
