The present invention relates to a specimen collecting device, including a first housing, which has a first chamber a fixing base accommodated in the first chamber of the first housing, in which the internal of the fixing base is sequentially provided with a first spring, a driving block, a piston, and a dual connecting rod from the bottom side to the outer side, the first spring being disposed between the bottom side of the fixing base and the driving block, the piston being disposed in the driving block, one end of the dual connecting rod being fixed to the piston, the other end of the dual connecting rod being fixed to the fixing base and provided with a firing button and a needle withdrawing button, wherein the firing button is fixed to the fixing base via a shaft member, and the fixing base includes a first oblique block and a second oblique block and a second housing connected with the first housing and having a second chamber that is provided therein with a connecting base, a needle withdrawing rod, a second spring, a firing base, and a collecting needle, in which one end of the needle withdrawing rod contacts the needle withdrawing button and the other end of the needle withdrawing rod penetrates the firing base. The second spring is provided between the needle withdrawing rod and the firing base. The firing base is connected with the collecting needle.本發明係一種檢體採集裝置,其係包括有:一第一殼體,具有一第一空腔;一固定座,用以容置於該第一殼體之該第一空腔中,該固定座內部由底側至外側依序設置有一第一彈簧、一驅動塊、一活塞及一雙連桿,其中該第一彈簧係設置於該固定座底側與該該驅動塊之間,該活塞係設置於該驅動塊中,該雙連桿一端固定於該活塞,該雙連桿之另一端固定於該固定座上,該雙連桿之該另一端更係設置有一擊發鍵及一退針鍵,該擊發鍵係利用一軸件固定於該固定座上,且該固定座係包括有一第一斜塊及一第二斜塊;一第二殼體,與該第一殼體做連接,具有一第二空腔,該第二空腔內設置有一連接座、一退針桿、一第二彈簧、一擊發座及一採集針,其中該退針桿一端係與該退針鍵接觸,且該退針桿之另一端穿置於該擊發座中,而該退針桿與該擊發座之間設置有該第二彈簧,該擊發座連接有該採集針。1‧‧‧第一殼體11‧‧‧第一空腔2‧‧‧固定座21‧‧‧擊發鍵22‧‧‧退針鍵23‧‧‧第一斜塊24‧‧‧第二斜塊3‧‧‧連接座4‧‧‧第二殼體51‧‧‧第一彈簧52‧‧‧驅動塊521‧‧‧滑槽53‧‧‧活塞54‧‧‧雙連桿55‧‧‧退針桿56‧‧‧第二彈簧57‧‧‧擊發座58‧‧‧採集針