PROCÉDÉ DE GÉNÉRATION DE CELLULES MÉSODERMIQUES ET/OU DE CELLULES DE TYPE CELLULES DE FORMATION DE COLONIES ENDOTHÉLIALES PRÉSENTANT UNE CAPACITÉ DE FORMATION DE VAISSEAUX SANGUINS IN VIVO
Indiana University Research & Technology Corporation
发明人:
YODER, Mervin C.,PRASAIN, Nutan
申请号：
EP17840057
公开号：
EP3497204A4
申请日:
2017.08.04
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
The present disclosure relates generally to methods and compositions useful in cell and tissue biology and therapeutics. In particular, an in vitro method for differentiating pluripotent stem cells into KDR+NCAM+APLNR+ mesoderm cells and/or SSEA5−KDR+NCAM+APLNR+ mesoderm cells is provided. The disclosed mesoderm cells may be used to generate blood vessels in vivo and/or further differentiated in vitro into endothelial colony forming cell-like cells (ECFC-like cells). Purified human cell populations of KDR+NCAM+APLNR+mesoderm cells and ECFC-like cells are provided. Test agent screening and therapeutic methods for using the cell populations of the present disclosure are provided.
