The present invention discloses a hygiene product with detection function and a manufacturing method thereof. The manufacturing method comprises the following steps: providing an impermeable adhesive bottom layer, disposing an absorption inner layer on the impermeable adhesive bottom layer, disposing a detection specimen layer on a central region of the absorption inner layer, covering a non-woven fixed layer on the detection specimen layer, disposing a synthetic absorption network layer on the non-woven fixed layer, and disposing a penetration surface layer on the synthetic absorption network layer.本發明係揭露一種具有檢測功能之衛生用品及其製造方法。該方法包含下列步驟︰提供一不滲透黏貼底層;設置一吸收內層於不滲透黏貼底層上;設置一檢測試片層於吸收內層之中央區域上;覆蓋一不織布固定層於檢測試片層上;設置一合成吸收網層於不織布固定層上;以及設置一滲透表層於合成吸收網層上。S11~S16...步驟