The present invention relates to a method for preparing a stem cell constituent extract and a method for preparing a hair loss treatment agent by using the same. The method for preparing a stem cell constituent extract, according to the present invention, comprises the steps of: obtaining cultured stem cells from which a medium has been removed counting the number of stem cells by dispersing the stem cells in an isotonic carrier liquid removing the carrier liquid and obtaining the stem cells dispersing the stem cells in a hypotonic extender of an amount determined by considering the number of stem cells and the target concentration and pulverizing the stem cells in the extender. The method for preparing a hair loss treatment agent, containing a stem cell constituent extract, according to the present invention, comprises the steps of: culturing stem cells in a culture solution washing the stem cells after removing the culture solution counting the number of stem cells by dispersing the stem cells in an isotonic carrier liquid removing the carrier liquid and obtaining the stem cells dispersing the stem cells in a hypotonic extender of an amount determined by considering the number of stem cells and the target concentration pulverizing the stem cells in the extender removing cell membrane residues and filtering out, from the stem cell extract, only a component having a molecular weight of 3,000-5,000.La présente invention concerne un procédé de préparation dun extrait de constituant de cellules souches et un procédé de préparation dun agent de traitement de la perte des cheveux à laide de celui-ci. Le procédé de préparation dun extrait de constituant de cellules souches, selon la présente invention, comprend les étapes consistant à: obtenir des cellules souches mises en culture à partir desquelles un milieu a été retiré compter le nombre de cellules souches en dispersant les cellules souches dans un liquide support isotonique retirer le liquide support et obtenir les c
