PROBLEM TO BE SOLVED: To provide cell cultures to differentiate pluripotent stem cells in vitro to endocrine cells which, after implantation into a mammal, produce insulin in response to glucose stimulation in vivo.SOLUTION: In vitro cell cultures contain human cells, in which at least 10% of the human cells is immature beta cells simultaneously expressing INS and NKX6.1, and Y-27632 in an amount effective to promote the survival of the immature beta cells in the in vitro cell cultures.SELECTED DRAWING: Figure 45【課題】多能性幹細胞を、哺乳動物に埋め込んだ後にｉｎ ｖｉｖｏでグルコース刺激に応答してインスリンを生成する内分泌細胞に、ｉｎ ｖｉｔｒｏにおいて分化させるための細胞培養物の提供。【解決手段】ヒト細胞であって、前記ヒト細胞の少なくとも１０％が、ＩＮＳおよびＮＫＸ６．１を同時発現する未成熟ベータ細胞である、ヒト細胞と、ｉｎ ｖｉｔｒｏ細胞培養物において前記未成熟ベータ細胞の生存を促進させる有効量のＹ－２７６３２とを含む、ｉｎ ｖｉｔｒｏ細胞培養物。【選択図】図４５
