SIMON ANTHONY JAMES WARNER,TIMOTHY ROBERT HA WKES,CHRISTOPHER JOHN ANDREWS
申请号：
IN6098/DELNP/2010
公开号：
IN2010DN06098A
申请日:
2010.08.27
申请国别(地区):
IN
年份:
2012
代理人:
摘要：
A method of selectively controlling weeds in a field, the field comprising weeds and plants, the method comprising application to the field of a glyphosate type herbicide in an amount sufficient to control the weeds without substantially affecting the plants, wherein said plants comprise a glyphosate resistant EPSPS enzyme said glyphosate resistant EPSPS enzyme characterized in that when said glyphosate resistant EPSPS enzyme is compared with the wild type EPSPS the protein sequence is modified in that a first position is mutated so that the residue at said first position is lie rather than Thr and a second position is mutated so that the residue at said second position is Ser rather than Pro, the mutations being introduced into EPSPS sequences which comprise the conserved region GNAGTAMRPL in the wild type enzyme such that the modified sequence reads GNAGIAMRSL, wherein the enzyme does not comprise the sequence K(A/V)AKRAVVVGCGGKFPVE, and wherein the enzyme is stable and possesses at least one of the following sequence motifs (i) to (viii) in which X is any amino acid and Z, Z1 and Z2are any amino acid other than those specified:- (i) ALLMZAPLA, wherein Z is not S or T； (ii) EIEIZDKL, wherein Z is not V； (iii) FG(V/I)(K/S)ZEH, wherein Z is not V； (iv) AL(K/R)ZLGL, wherein Z is not R； (v) GLXVEZ1DXZ2XXXA(l/V)V, wherein Zx is not T and/or Z2 is not E； (vi) ITPPZ1K(L/V)(K/N)Z2, wherein Z1 is not K and/or Z2 is not T； (vii) TIZ(D/N)PGCT, wherein Z is not N or L； (viii) .(D/N)YFXVLXZXX(K/R)H, wherein Z is not R.
