The invention provides a method for decellularising a tissue the method comprising the steps of: a) adding a tissue to be decellularised to a decellularisation vessel； b) adding a decellularisation medium comprising at least one detergent and/or at least one enzyme to the vessel； c) introducing a supercritical fluid to the vessel； and d)maintaining contact of the tissue with the supercritical fluid for a time sufficient to substantially decellularise the tissue.
