The present invention discloses a dual-targeting drug carrier and a method for fabricating the same, wherein WGA- and FA-modified MPEG-PLA nanoparticles of the carrier enable the anticancer drugs encapsulated thereinside to pass through BBB and target human glioblastoma cells. The dual-target drug carrier is fabricated in an emulsion-solvent evaporation technology and verified with an in-vitro BBB model formed of HBMECs, HAs and HBVPs. The present invention can increase the permeability of the in-vitro BBB model to the dual-target drug carrier and promote the glioblastoma-inhibition effect. Therefore, the present invention would contribute to the clinical therapy of brain cancers substantially in the future.本發明揭露一種雙標靶藥物釋放載體及其製作方法,其雙標靶藥物釋放載體乃是以麥胚凝集素和葉酸修飾聚乙二醇聚己內脂奈米粒子表面,使聚乙二醇聚己內脂奈米粒子所包覆的抗癌藥物能夠穿透血腦屏障,並標靶人類惡性膠質母細胞。此雙標靶藥物釋放載體可由微乳化溶劑揮發法來製備,且本發明係利用人腦微血管內皮細胞、人類星形細胞及人類周細胞建立人類生體外血腦屏障模型,證明此雙標靶藥物釋放載體能夠提高對人類生體外血腦屏障的穿透率,及增加對人類惡性膠質母細胞瘤的抑制,藉此,對於未來臨床上腦腫瘤的治療將可望提供實質上的幫助。
