PROBLEM TO BE SOLVED: To provide an organism insertion device and a puncture method which can reliably apply a surgical treatment to a biological tissue positioned between a urethral lumen and a vaginal cavity.SOLUTION: An insertion tool 6 comprises: a urethra insertion member 4 with a long length to be inserted to a urethra 1300 a vagina insertion member 5 with a long length to be inserted to a vagina 1400 and a supporting part 60 which interconnects the urethra insertion member 4 and the vagina insertion member 5 to be capable of approaching and separating from each other. The supporting part 60 comprises: a first locking part 64 which keeps a state where the urethra insertion member 4 and the vagina insertion member 5 have approached each other and a second locking part 65 which keeps a state where the urethra insertion member 4 and the vagina insertion member 5 are away from each other.COPYRIGHT: (C)2015,JPO&INPIT【課題】尿道腔と膣腔との間に位置する生体組織に対して外科的処置を施す際、その処置を確実に行なうことができる生体挿入装置および穿刺方法を提供すること。【解決手段】挿入具6は、尿道1300に挿入される長尺状をなす尿道挿入部材4と、膣1400に挿入される長尺状をなす膣挿入部材5と、尿道挿入部材4と膣挿入部材5とを接近・離間可能に連結する支持部60とを備え、支持部60は、尿道挿入部材4と膣挿入部材5とを接近させた状態を維持する第1のロック部64と、尿道挿入部材4と膣挿入部材5とを離間させた状態を維持する第2のロック部65とを有する。【選択図】図58