Provided is a method of activating osteogenic or chondrogenic activity at a site in a subject in need thereof. Also provided is a method of treating a bone or cartilage defect in a subject. Additionally, the use of a reduced pressure apparatus for treating a bone or cartilage defect adjacent to dura mater, periosteum, or endosteum is provided. Further provided is a composition for treating a bone or cartilage defect. Also, the use of a reduced pressure apparatus and a biocompatible scaffold for the manufacture of a composition for treating a bone or cartilage defect adjacent to dura mater, periosteum or endosteum is provided.本發明提供一種在有其需要之個體某處部位活化成骨性或成軟骨活性之方法。本發明亦提供一種治療個體骨骼或軟骨缺陷之方法。另外,本發明提供利用減壓儀器以治療鄰近硬腦膜、骨膜或骨內膜之骨骼或軟骨缺陷。本發明進一步又提供用以治療骨骼或軟骨缺陷之組合物。另外,本發明提供利用減壓儀器及生物相容性支架以製造供治療鄰近硬腦膜、骨膜或骨內膜之骨骼或軟骨缺陷之組合物的用途。10‧‧‧系統14‧‧‧減壓源18‧‧‧歧管及/或支架22‧‧‧硬腦膜30‧‧‧濾毒罐34‧‧‧導管
