Durack, Gary,Ludwig, Cindy L.,Graham, Jeffrey A.,Crowley, Kathleen S.,Hatcher, Jeremy T.,Westfall, Lon A.,Helbing, David R.,Wallace, Jeffrey D.,Vandre, Gary P.,Didion, Bradley,Nayak, Niraj V.,Anzar, M
申请号：
ES10184098
公开号：
ES2586482T3
申请日:
2004.03.29
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
Apparatus for classification by sex of sperm cells in a fluid stream according to one or more characteristics of the sperm cells, said system comprising: an apparatus for flow cytometry to provide a fluid stream containing said sperm cells to a first location and to cause current to break up into droplets at a second location, said apparatus cytometry operational flow using flow cytometry to sort the sperm cells according to these characteristics and to sort said droplets by classification of sperm cells contained in the droplets wherein the apparatus further comprises flow cytometry a nozzle having an inner surface configured to exert a force on the sperm cells that tends to bring them into a desired orientation before passing through said beam and said apparatus comprising flow cytometric optical system epi-illumination includes a focusing lens, said optical system operable to direct a laser beam through said focusing lens in a forward direction along an axis beam crossing the fluid flow in said first location so that said sperm cells pass through the beam, producing electromagnetic radiation emissions of sperm cells directed along said beam axis in a reverse direction, the wherein said optical system includes a single photodetector at the back of said focusing lens for detecting said emissions, wherein said beam is focused by said optical system as a point on the fluid stream at said first location, said point one elliptical shape with a length along a major axis extending at right angles to the direction of flow d and the fluid stream and a width along a minor axis which extends parallel to the flow direction of the fluid stream, said smaller width than the length of the head of a sperm cell passing through point .Aparato para la clasificación por sexo de células espermáticas contenidas en una corriente de fluido según una o más características de las células espermáticas, comprendiendo dicho sistema: un aparato de citometría de flujo para suministrar una corrie
