The current invention provides a method for ruling out acute myocardial infarction (AMI) in a subject presenting with chest pain suspected to be cardiac in nature. The method includes a) recording an Electrocardiography (ECG) reading from said subject, b) determining the amount of heart-type fatty acid binding protein (H-FABP) and cardiac Troponin T (cTnT) in a sample from said subject, c) comparing the results from b) to reference values for ruling out an AMI in a subject, and d) based on the results from steps a) - c) either ruling out or ruling in a diagnosis of AMI in said subject.
